There were two pots of clubs for the round of 32 draw: the 12 group winners and the four best third-ranked teams in the Champions League group stage were seeded. These teams will play the second leg at home.
The 12 group runners-up and the remaining third-placed Champions League sides made up the second pot, playing the return fixture away. No team could be paired with a club from their Europa League group or a club from the same country.
Former Lille, Lyon and Barcelona defender Eric Abidal was welcomed on to the stage to help out with the draw.
The ties will be played in principle on Thursdays 15 and 22 February, at 19:00 and 21:05, and Lyon is the venue for the final of the Europa League on May 16.
BORUSSIA DORTMUND V ATALANTA
SPARTAK MOSCOW V ATHLETIC BILBAO
FC COPENHAGEN V ATLETICO MADRID
NICE V LOKOMOTIV MOSCOW
STEAUA BUCHAREST V LAZIO
PARTIZAN BELGRADE V VIKTORIA PLZEN
REAL SOCIEDAD V SALZBURG
LYON V VILLARREAL
RED STAR BELGRADE V CSKA MOSCOW
NAPOLI V LEIPZIG
CELTIC V ZENIT
AEK ATHENS V DYNAMO KIEV
MARSEILLE V BRAGA
OSTERSUNDS V ARSENAL
ASTANA V SPORTING LISBON
LUDOGORETS V AC MILAN